Fig 1: miR376c-Mediated Gene Silencing Is Reduced after iLTP(A) qRT-PCR showing miRNA expression levels following iLTP induction (normalized to U6 snRNA). Colored stars compare statistical significance to the ctrl; n = 4.(B) Luc-GABRA1 activity in neurons under control conditions or 90 min post-iLTP induction; n = 5.(C) As in (B) but for Luc-GABRG2; n = 5(D) Luc-GABRA1 activity in neurons expressing anti-miRCon or anti-miR376c, under control conditions or 90 min post-iLTP induction; n = 5.(E) As in (D), but for Luc-GABRG2; n = 5.(F) a1, ?2, and gephyrin levels 90 min post-iLTP induction in neurons expressing miRCon or miR376c; n = 6.(G) GFP-filled dendritic segments from miRNA overexpressing hippocampal neurons labeled with antibodies to surface GABAAR?2 and gephyrin or VGAT. Scale bar, 10 µm.(H) Quantification of cluster area and density from (E); n = 16–18 neurons per condition. Colored stars compare statistical significance with ctrl.(I) mIPSC traces from neurons infected with miRCon or miR376c. Graphs show mIPSC amplitude and frequency; n = 9–13 cells per condition.All values represent mean ± SEM. *p < 0.05 and **p < 0.01, one-way ANOVA, Bonferroni post hoc test (A) and two-way ANOVA, Bonferroni post hoc test (B–F and I).